Hi, thanks for your feedback!
This kind of issue should have been fixed as of version 1.0.5 of the addon. It was reported to me in ticket #3, and the issue seem to persist only with files created with older Blender versions, in which case I recommend trying to make a new clean file from the latest Blender version and then append the content of your old file in there.
If you still encounter issues, please send a bug report on this link:
https://github.com/L0Lock/AudioNotifier/issues/new/choose
Great addon and a very good idea, it's especially useful to hear a sound alert when the render finishes. However, for it to work, I have to disable/enable the addon every time I open Blender (ver. 4.4.3).